SGMS2 Stable Expression Cell Line (NCI-H1299)

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  • 细胞图0 SGMS2 Stable Expression Cell Line (NCI-H1299)-细胞图0
  • 细胞图1 SGMS2 Stable Expression Cell Line (NCI-H1299)-细胞图1
  • 质粒图0 SGMS2 Stable Expression Cell Line (NCI-H1299)-质粒图0
  • SGMS2 Stable Expression Cell Line (NCI-H1299) SGMS2 Stable Expression Cell Line (NCI-H1299)-产品图
细胞图0 SGMS2 Stable Expression Cell Line (NCI-H1299)-细胞图0
细胞图1 SGMS2 Stable Expression Cell Line (NCI-H1299)-细胞图1
质粒图0 SGMS2 Stable Expression Cell Line (NCI-H1299)-质粒图0
SGMS2 Stable Expression Cell Line (NCI-H1299) SGMS2 Stable Expression Cell Line (NCI-H1299)-产品图
SGMS2 Stable Expression Cell Line (NCI-H1299) Copy Product Info
Catalog#： YOE-L081
Size： 1*10^6
Culture method： 88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
STR Report
Datasheet (Use Instruction)
  • 细胞图0 SGMS2 Stable Expression Cell Line (NCI-H1299)-细胞图0
  • 细胞图1 SGMS2 Stable Expression Cell Line (NCI-H1299)-细胞图1
  • 质粒图0 SGMS2 Stable Expression Cell Line (NCI-H1299)-质粒图0
  • SGMS2 Stable Expression Cell Line (NCI-H1299) SGMS2 Stable Expression Cell Line (NCI-H1299)-产品图
SGMS2 Stable Expression Cell Line (NCI-H1299) Copy Product Info
Catalog#： YOE-L081
Size： 1*10^6
Culture method： 88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Catalog YOE-L081 Transcript NM_152621.6
Cell name SGMS2 Stable Expression Cell Line (NCI-H1299) Species Human
Gene Name SGMS2 Passage ratio 1:2~1:3
Cell Morphology Epithelial-like, adherent Antibiotic Concentration Puro=2μg/ml
Culture method 88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate Mycoplasma test negative
Catalog
YOE-L081
Cell name
SGMS2 Stable Expression Cell Line (NCI-H1299)
Gene Name
SGMS2
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Transcript
NM_152621.6
Species
Human
Antibiotic Concentration
Puro=2μg/ml
Mycoplasma test
negative
Culture method
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate

Product Description

Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene. There is evidence for more variants, but the full-length nature of their transcripts has not been determined.
Gene Expression Validation
No. Sample hSGMS2-1CтMean β-h_actinCтMean ΔCтMean ΔΔCт Expression Rate
1 SGMS2 Stable Expression Cell Line (NCI-H1299) 16.511 14.797 1.714 -5.884 59.056
2 NCI-H1299_Ctrl(Puro) 22.613 15.015 7.598 0.000 1.000
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H1299		 STR Info (Cell bank)
Cell Line:NCI-H1299-Luc2-tdT-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 12121212
D7S820 10101010
D16S539 12131213
VWA 16181618
TH01 69.369.3
AMEL XXXX
TPOX 8888
CSF1PO 12121212
D12S391 2122
FGA 2020
D2S1338 2324
D21S11 32.232.2
D18S51 1616
D8S1179 1013
D3S1358 1717
D6S1043 1111
PENTAE 1111
D19S433 1414
PENTAD 1313
D1S1656 1215
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    High expression
    Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
  • icon
    Good stability
    Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
  • icon
    Strict QC
    Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
Learn More>>>
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